ABSTRACT
Fast tract surgery (FTS) has been implemented in different fields of surgery to attenuate the surgical stress response and accelerate recovery.Liver resection is the preferred treatment for a variety of primary and secondary liver tumors.However,liver resection is associated with severe stress response and higher rate of postoperative morbidity and mortality.Fast tract surgery has been reported to accelerate recovery following liver resection.In this review,we summarize the recent progress of fast tract surgery in liver resection.
ABSTRACT
Objective To develop an appropriate and simple decellularization protocol for preparing decellularized liver bio-logical scaffold(DLBS) .Methods Through immune-ofluorescence analysis ,scanning electron microscope ,quantification of Glyco-samin-oglycans(GAGs) and DNA ,we evaluated the effectiveness of low concentrations of SDS(0 .25% ,0 .50% ) and 1% Triton X-100 in the preservation of extracellular matrix of rat livers .Then ,we assessed Cytotoxicity of DLBS treated with three methods by MTT assay .Results Residual DNA after SDS(0 .25% ,0 .50% ) treatments were below 50 ng/mg dry weight ,which were less than Triton X-100 treatments .The content of GAGs in the 0 .25% SDS and 1% Triton X-100-treated scaffolds were higher than other detergent-treated scaffolds .The result of MTT assay showed that the liver scaffolds treated with three methods were not cytotoxic to proliferation of C3A cells .Conclusion The protocol containing 0 .25% SDS and a perfusion rate of 5 mL/min could be a rapid and effective decellularization protocol .DLBS constructed using this protocol could be an ideal material for preparing a transplant-able organ .
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of single-incision laparoscopic cholecystectomy using a three-dimensional (3D) laparoscopic system.</p><p><b>METHODS</b>Two patients with benign gallbladder disease with a history of recurrent abdominal pain were selected to undergo the surgery. Gallstones were diagnosed by B ultrasound examination. All the operations were performed through the umbilical incision with the 3D laparoscopic system.</p><p><b>RESULTS</b>The 2 operations were completed successfully with a operative time of 35 min and 50 min. Both of the patients were ambulatory 8 h after the surgery, began to have a normal diet 1 day after operation, and were discharged 2 days postoperatively without any clinical symptoms.</p><p><b>CONCLUSIONS</b>3D single-incision laparoscopic operation can well reveal the tissue anatomy in the operative field especially for some important structures such as the Calot's triangle with an improved safety compared to conventional laparoscopic operations.</p>